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  • P-ISSN2233-4203
  • E-ISSN2093-8950

Dynamic MRM Measurements of Multi-Biomarker Proteins by Triple- Quadrupole Mass Spectrometry with Nanoflow HPLC-Microfluidics Chip

Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2010, v.1 no.1, pp.21-24
https://doi.org/10.5478/MSL.2010.1.1.021
Eun Sun Ji (Korea Basic Science Institute,Hannam University)
Mi Hee Cheon (Korea Basic Science Institute,Yonsei University)
Ju Yeon Lee (Korea Basic Science Institute,)
Jong Shin Yoo (Korea Basic Science Institute, Chungnam National University)
Hyun-Jin Jung (Agilent technologies Korea Ltd.)
Jin Young Kim (Korea Basic Science Institute)
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Abstract

The development of clinical biomarkers involves discovery, verification, and validation. Recently, multiple reactionmonitoring (MRM) coupled with stable isotope dilution mass spectrometry (IDMS) has shown considerable promise for the directquantification of proteins in clinical samples. In particular, multiple biomarkers have been tracked in a single experiment usingMRM-based MS approaches combined with liquid chromatography. We report here a highly reproducible, quantitative, anddynamic MRM system for validating multi-biomarker proteins using Nanoflow HPLC-Microfluidics Chip/Triple-QuadrupoleMS. In this system, transitions were acquired only during the retention window of each eluting peptide. Transitions with the highestMRM-MS intensities for the five target peptides from colon cancer biomarker candidates were automatically selected usingOptimizer software. Relative to the corresponding non-dynamic system, the dynamic MRM provided significantly improvedcoefficients of variation in experiments with large numbers of transitions. Linear responses were obtained with concentrationsranging from fmol to pmol for five target peptides.

Submission Date
2010-11-14
Revised Date
2010-11-27
Accepted Date
2010-11-27

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Mass Spectrometry Letters