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- E-ISSN 2093-8950
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Electron Capture Dissociation Mass Spectrometry for Gaseous Protonated Melittin Ions and Its Single Amino Acid Substituted Variants
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2019, v.10 no.4, pp.117-122
https://doi.org/10.5478/MSL.2019.10.4.117
(Sogang University)
(Sogang University)
(Sogang University)
(Sogang University)
(Sogang University)
Yu,
S., Jang,
H., &
Oh,
H.
B.
(2019). Electron Capture Dissociation Mass Spectrometry for Gaseous Protonated Melittin Ions and Its Single Amino Acid Substituted Variants. Mass Spectrometry Letters, 10(4), 117-122, https://doi.org/10.5478/MSL.2019.10.4.117
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Abstract
This study demonstrated the sensitivity of electron capture dissociation mass spectrometry (ECD-MS) to probe sub- tle conformational changes in gaseous melittin ions induced by the substitution of an amino acid. ECD-MS was performed for triply and quadruply-protonated melittin and its variants obtained by a single amino acid substitution, namely, D-Pro14, Pro14Ala, and Leu13Ala. Although native triply-protonted melittin showed only a few peptide backbone cleavage products, the D-Pro14 and Pro14Ala variants exhibited extensive backbone fragments, suggesting the occurrence of a significant structural or conformational change induced by a single amino acid substitution at Pro14. On the contrary, the substitution at Leu13, namely Leu13Ala (+3), did not cause significant changes in the ECD backbone fragmentation pattern. Thus, the sensitivity of ECD-MS is demonstrated to be good enough to probe the aforementioned conformational change in melittin.
- keywords
- Electron Capture Dissociation, Melittin, Amino Acid Substitution, Chiral Inversion, Mass Spectrometry
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- Submission Date
- 2019-12-14
- Revised Date
- 2019-12-24
- Accepted Date
- 2019-12-24
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