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  • P-ISSN2233-4203
  • E-ISSN2093-8950

Determination of Mertansine in Rat Plasma Using Liquid Chromatography-Tandem Mass Spectrometry and Pharmacokinetics of Mertansine in Rats

Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2020, v.11 no.3, pp.0-0
https://doi.org/10.5478/MSL.2020.11.3.59
Won-Gu Choi (The Catholic University of Korea)
Ju-Hyun Kim (Yeungnam University)
Hyun-Joon Jang (The Catholic University of Korea)
Hye Suk Lee (The Catholic University of Korea)

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Abstract

Mertansine, a thiol-containing maytansinoid, is a tubulin inhibitor used as the cytotoxic component of antibody-drug conjugates for the treatment of cancer. Liquid chromatography-tandem mass spectrometry was described for the determination of mertansine in rat plasma. 50-µL rat plasma sample was pretreated with 25 µL of 20 mM tris-(2-carboxyethyl)-phosphine, a reducing reagent, and further vortex-mixing with 50 µL of 50 mM N-ethylmaleimide for 3 min resulted in the alkylation of thiol group in mertansine. Alkylation reaction was stopped by addition of 100 µL of sildenafil in acetonitrile (200 ng/mL), and following centrifugation, aliquot of the supernatant was analyzed by the selected reaction monitoring mode. The standard curve was linear over the range of 1–1000 ng/mL in rat plasma with the lower limit of quantification level at 1 ng/mL. The intra- and inter-day accuracies and coefficient variations for mertansine at four quality control concentrations were 96.7–113.1% and 2.6–15.0%, respectively. Using this method, the pharmacokinetics of mertansine were evaluated after intravenous administration of mertansine at doses of 0.2, 0.5, and 1 mg/kg to female Sprague Dawley rats.

Submission Date
2020-08-20
Revised Date
2020-09-06
Accepted Date
2020-09-09

Mass Spectrometry Letters