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  • P-ISSN2233-4203
  • E-ISSN2093-8950

Analysis of Hypoxia-Inducible Factor Stabilizers by a Modified QuEChERS Extraction for Antidoping Analysis

Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2020, v.11 no.4, pp.118-124
https://doi.org/10.5478/MSL.2020.11.4.118
Si Hyun Kim (Korea Institute of Science and Technology)
Nu Ri Lim (Korea Institute of Science and Technology)
Hophil Min (Korea Institute of Science and Technology)
Changmin Sung (Korea Institute of Science and Technology)
Han Bin Oh (Sogang University)
Ki Hun Kim (Korea Institute of Science and Technology)

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Abstract

An analytical method was developed for hypoxia-inducible factor (HIF) stabilizers based on QuEChERS (Quick, Easy, Cheap, Effective, Rugged, and Safe) sample preparation and liquid chromatography–high resolution mass spectrometry analysis. HIF stabilizers potentially enhance the performance of athletes, and hence, they have been prohibited. However, the analysis of urinary HIF stabilizers is not easy owing to their unique structure and characteristics. Hence, we developed the QuEChERS preparation technique for a complementary method and optimized the pH, volume of extraction solvent, and number of extractions. We found that double extraction with 1% of formic acid in acetonitrile provided the highest recovery of HIF stabilizers. Moreover, the composi-tion of the mobile phase was also optimized for better separation of molidustat and IOX4. The developed method was validated in terms of its precision, detection limit, matrix effect, and recovery for ISO accreditation. To the best of our knowledge, this is the first demonstration of the application of the QuEChERS method, which is suitable as a complementary analytical method, in antidoping.

Submission Date
2020-12-18
Revised Date
2020-12-30
Accepted Date
2020-12-30

Mass Spectrometry Letters