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- E-ISSN 2093-8950
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Identfication of Phase I and Phase II Metabolites of Hesperetin in Rat Liver Microsomes by Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2011, v.2 no.1, pp.20-23
https://doi.org/10.5478/MSL.2011.2.1.020
(Chung-Ang university, Korea institute of Science and Technology)
(Chung-Ang university)
(Korea Institute of Science and Technology)
(Hanyang University)
(Chung-Ang university)
(Korea Institute of Science and Technology)
(Hanyang University)
Kim,
U., Han,
S. B., Kwon,
O., &
Yoo,
H.
H.
(2011). Identfication of Phase I and Phase II Metabolites of Hesperetin in Rat Liver Microsomes by Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry. Mass Spectrometry Letters, 2(1), 20-23, https://doi.org/10.5478/MSL.2011.2.1.020
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Abstract
The purpose of this study is to investigate the in vitro metabolism of hesperetin, a bioflavonoid. Hesperetin was incubatedwith rat liver microsomes in the presence of NADPH and UDP-glucuronic acid for 30 min. The reaction mixture was analyzedby liquid chromatography-ion trap mass spectrometer and the chemical structures of hesperetin metabolites were characterzed basedon their MS/MS spectra. As a result, a total of five metabolites were detected in rat liver microsomes. The metabolites were identifiedas a demethylated metabolite (eriodictyol), two hesperetin glucuronides, and two eriodictyol glucuronides.
- keywords
- Hesperetin, in vitro Metabolism, Rat Liver Microsomes, LC/MS
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- Submission Date
- 2011-02-23
- Revised Date
- 2011-03-12
- Accepted Date
- 2011-03-12
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