- P-ISSN 2233-4203
- E-ISSN 2093-8950
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- E-ISSN2093-8950
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Accurate Quantification of Saccharin Using Isotope Dilution Liquid Chromatography Mass Spectrometry (ID-LC/MS)
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2011, v.2 no.2, pp.37-40
https://doi.org/10.5478/MSL.2011.2.2.037
(Korea Research Institute of Standards and Science)
(Korea Research Institute of Standards and Science)
(Chungnam National University)
(Korea Research Institute of Standards and Science)
(Korea Research Institute of Standards and Science)
(Chungnam National University)
(Korea Research Institute of Standards and Science)
Lee,
Y., Kim,
B., Kim,
J., &
Ahn,
S.
(2011). Accurate Quantification of Saccharin Using Isotope Dilution Liquid Chromatography Mass Spectrometry (ID-LC/MS). Mass Spectrometry Letters, 2(2), 37-40, https://doi.org/10.5478/MSL.2011.2.2.037
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Abstract
Saccharin is a commonly used artificial sweetener in foodstuffs. However, for its carcinogenic dispute, it has beenregulated by government bodies. In this study, isotope dilution mass spectrometry (ID-MS) was introduced for the accuratequantification of saccharin. To employ ID-LC/MS, we obtained its isotope analogue, 13C1−sodium saccharin, by customized synthesis. Samples were spiked with 13C1-sodium saccharin and analyzed with LC/MS in negative mode. Chromatographic conditionswere optimized for the adequate chromatographic retention and separation of saccharin with a C18 column. MS was operatedwith electrospray ionization by the selected ion monitoring (SIM) mode of [M − H]− for saccharin (m/z 182) and [M − Na]− forits isotope analogue (m/z 183). To validate the ID-LC/MS method for accurate measurement, we prepared a batch of a candidatematerial by sortifying quasi−tea−drinks with saccharin and analyzed samples gravimetrically fortified in various levels of concentration. The repeatability and reproducibility of this method was tested by analyzing the reference material. Result show thatID-LC/MS is a reliable method for the quantitative analysis of saccharin.
- keywords
- Saccharin, Isotope analogue-13C1-sodium saccharin, Isotope dilution mass spectrometry (ID-MS), Repeatability, Reproducibility
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- Submission Date
- 2011-05-11
- Revised Date
- 2011-06-03
- Accepted Date
- 2011-06-08
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