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- E-ISSN 2093-8950
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- P-ISSN2233-4203
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Microwave-assisted Protein Digestion on Various Locations of a Microplate
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2011, v.2 no.4, pp.84-87
https://doi.org/10.5478/MSL.2011.2.4.084
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Korea Basic Science Institute)
(Korea Basic Science Institute)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Chungnam National University)
(Korea Basic Science Institute)
(Korea Basic Science Institute)
(Chungnam National University)
Lee,
J. H., Park,
S., Lee,
S., Hong,
J., Park,
K. H., Hyun Sik,
K., &
Kim,
J.
(2011). Microwave-assisted Protein Digestion on Various Locations of a Microplate. Mass Spectrometry Letters, 2(4), 84-87, https://doi.org/10.5478/MSL.2011.2.4.084
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Abstract
The effectiveness of microwave-assisted protein digestion in different well positions of a 96-well microplate wasinvestigated where microwave-assisted protein digestion of bovine serum albumin was performed in 10 different wells of a 96-wellmicroplate in a microwave oven. Similarly increased sequence coverages (~70%) were generally observed for the 10 microwaveassistedprotein digestion samples compared to conventional overnight digestion (63%), which is possibly due to higher miscleavageratios (~53%) of the samples from microwave-assisted protein digestion than conventional overnight digestion (42.1%). The reproducibleresults of microwave-assisted digestions from different well positions demonstrate the potential of high-throughput analysis ofproteins using microwave-assisted protein digestion.
- keywords
- Microwave, Mass spectrometry, MALDI, Microplate, Digestion
Reference
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- Submission Date
- 2011-12-09
- Revised Date
- 2011-12-13
- Accepted Date
- 2011-12-14
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