Fat malabsorption is an important cause of poor growth in infancy and childhood. Steatorrhea tests have been developedusing various methods. Traditional measurements of stool fat, however, require large samples and it often takes as a week tocomplete the analysis. In this paper, a liquid chromatography-electrospray ionization/mass spectrometry (LC-ESI/MS) methodwas developed for simultaneous quantitative analysis of triacylglycerols, triolein, diolein and monoolein, in mouse feces. Moreover,the procedure was rapid, simple as well as compatible with LC-ESI/MS. Chloroform-isopropyl alcohol solution was usedfor fat-soluble sample extraction. After centrifugation and filtration, an analytical solution was prepared. Triolein, diolein andmonoolein were separated using non-aqueous reversed-phase column with the mobile phase consisting of A (methanol) and B(acetone-isopropyl alcohol). The precision (% CV) and accuracy (% bias) of the assay were 3.8-14.7% and 85.2-114.9%, respectively. This method has been successfully applied to simultaneous determination of triolein, diolein and monoolein in feces from30 mice. This method can therefore be applied to measure triacylglycerols in mouse feces accurately and precisely by LC-ESI/MS, thereby helping to predictive biomarker in fat malabsorption and diagnostic research.
Organic acid (OA) profiling analysis was performed in culture media from Lactobacillus pentosus K34 (L. pentosusK34) and Pediococcus lolli PL24 (P. lolli PL24) by gas chromatography-mass spectrometry (GC-MS) following methoxime/tert-butyldimethylsilyl derivatives. 12 OAs were positively identified in culture media. Most of OA levels from L. pentosus K34of hetero lactic fermentation were found to be higher when compared with those from P. lolli PL24 of homo lactic fermentation,which may explain different OA metabolism in each strain. In addition, the distorted dodecagonal star patterns were readily distinguishable,and the characteristics of each strain were well represented. The present study demonstrates that the OA metabolicprofiling method by GC-MS combined with star pattern recognition is useful for the monitoring study of characteristic OAmetabolism in various microorganisms.
The hydrophobic hydrocarbon chain and the polar sulfate group confer surfactant properties and enable them to beused as anionic surfactants. Anionic surfactants (AS) are known for their adverse impact on environment, particularly on aquaticecosystem. In the present study a fast, sensitive and selective method for the determination and subsequent quantification of sixanionic surfactants was developed using hydrophilic interactive liquid chromatography (HILIC) coupled to a electrospray ionization(ESI) mass spectrometer (MS), in the concentration range 15-20 μg/L. The capability of the method was establishedusing regression analysis and ANOVA. The method performance was evaluated by analyzing real time surface water spiked with1-dodecyl hydrogen sulfate at 15 μg/L. Combined efficiency of solid phase extraction and MS detection established recovery of89% in presence of natural matrix. These results point out that HILIC coupled to multistage MS procedures can be a powerfultechnique for environmental applications concerning the screening of polar contaminants.
The formation of zinc finger peptide−Zn2+ complexes in electrospray ionization mass spectrometry (ESI-MS) wasexamined using three different metal ion sources: ZnCl2, Zn(CH3COO)2, and Zn(OOC(CHOH)2COO). For the four zinc fingerpeptides (Sp1-1, Sp1-3, CF2II-4, and CF2II-6) that bind only a single Zn2+ in the native condition, electrospray of apo-zinc fingerin solution containing ZnCl2 or Zn(CH3COO)2 resulted in the formation of zinc finger−Zn2+ complexes with multiple zincions. This result suggests the formation of nonspecific zinc finger−Zn2+ complexes. Zn(tartrate), Zn(OOC(CHOH)2COO),mainly produced specific zinc finger−Zn2+ complexes with a single zinc ion. This study clearly indicates that tartrate is an excellentcounter ion in ESI-MS studies of zinc finger−Zn2+ complexes, which prevents the formation of nonspecific zinc finger−Zn2+complexes.