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  • P-ISSN 2233-4203
  • E-ISSN 2093-8950

Development of a Four-way Interface for Online Capillary Isoelectric Focusing-Electrospray-Mass Spectrometry (CIEF-ESI-MS)

Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2013, v.4 no.4, pp.83-86
https://doi.org/10.5478/MSL.2013.4.4.83
Yu Hai Dong (Korea Research Institute of Standard and Science)
Kim Byungjoo (Korea Research Institute of Standard and Science)
Shin Dae-Ho
Ahn Seonghee (Korea Research Institute of Standard and Science)
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Abstract

A new interface for coupling CIEF and MS using a four-way cross has been developed in a single mechanical system. This new interface could be operated without the electric discontinuity and reinstallation of lines. Additionally, a bare fused silicacapillary was facilitated as a spray needle to produce electrospray and to guide catholyte or sheath liquid. Focusing for CIEFwas completed in a hanging droplet at the end of spray needle. This capillary spray needle also provided stable spray, enhancedthe ionization efficiency and increased sensitivity. Results with carbonic anhydrase I showed that focusing and spraying werewell completed with the new interface and the new spray needle.

keywords
CIEF-ESI-MS, Interface, Carbonic anhydrase I, Fourier transform mass spectrometry


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Submission Date
2013-12-11
Revised Date
2013-12-19
Accepted Date
2013-12-19
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