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  • P-ISSN2233-4203
  • E-ISSN2093-8950

Phospholipid Analysis by Nanoflow Liquid Chromatography-Tandem Mass Spectrometry

Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2014, v.5 no.1, pp.1-11
https://doi.org/10.5478/MSL.2014.5.1.1
Myeong Hee Moon (Yonsei University)
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Abstract

Lipids play important roles in biological systems; they store energy, play a structural role in the cell membrane, and areinvolved in cell growth, signal transduction, and apoptosis. Phospholipids (PLs) in particular have received attention in the medical andlipidomics research fields because of their involvement in human diseases such as diabetes, obesity, atherosclerosis, and many cancersassociated with lipid metabolic disorders. Here I review experimental strategies for PL analysis based on nanoflow liquid chromatography-electrospray ionization-tandem mass spectrometry (nLC-ESI-MSn). In particular, discussed are lipid extraction methods, nanoflowLC separation of PLs, effect of ionization modifiers on the ESI of PLs, influence of chain lengths and unsaturation degree of acyl chainsof PLs on MS intensity, structural determination of the molecular structure of PLs and their oxidized products, and quantitative profilingof PLs from biological samples such as tissue, urine, and plasma in relation to cancer and coronary artery disease.

Submission Date
2014-02-05
Revised Date
2014-02-25
Accepted Date
2014-02-25

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Mass Spectrometry Letters