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  • P-ISSN2233-4203
  • E-ISSN2093-8950

Vol.6 No.1

Huwei Liu(Peking University) ; Xianjiang Li(Peking University) ; Xin Wang(Peking University) ; Linnan Li(Peking University) ; Yu Bai(Peking University) pp.1-6 https://doi.org/10.5478/MSL.2015.6.1.1
초록보기
Abstract

Direct analysis in real time mass spectrometry (DART-MS) is one of the variants of ambient mass spectrometry. Theionization process of DART-MS is in open environment and only takes few seconds, so it is suitable for fast analysis. Actually,since its introduction in 2005, more and more attentions have been drawn to its various applications due to its excellent properties,e.g., fast analysis, and no or less sample preparation, high salt tolerance and so on. This review summarized the promisingfeatures of DART-MS, including its ionization mechanism, equipment modification, wide applications, coupling techniques andextraction strategies before analysis.

Choong Sik Lee(Sogang University, Ministry of National Defense) ; Soo jin Park(Sogang University) ; Jae Young Lee(Dongguk University) ; Sungsu Park(Sungkyunkwan University) ; Kyubong Jo(Sogang University) ; Han Bin Oh(Sogang University) pp.7-12 https://doi.org/10.5478/MSL.2015.6.1.7
초록보기
Abstract

In the present study, we proposed a simple ESI-MS model for determining Zn2+ binding (or dissociation) constants forzinc finger peptides (ZFPs) with a unique ββα fold consensus. The ionization efficiency (response) factors for this model, i.e., α andβ, could be determined for ZiCo ZFP with a known Zn2+ binding constant. We could determine the binding constants for other ZFPsassuming those with a ββα consensus conformation have the same α/β response ratio. In general, the ZPF dissociation constantsexhibited Kd values of 10-7~10-9 M, while Kd values for a negative control non-specific Zn2+peptides were high, e.g., 5.5×10-6 M and4.3×10-4 M for BBA1 and melittin, respectively.

YanXuan Fan(Beijing Institute of Technology) ; Yan Zhang(Beijing Institute of Technology) ; QiaoDi Jia(Beijing Institute of Technology) ; Jie Cao(Beijing Institute of Technology) ; WenJie Wu(Tianjin University of Science and Technology) pp.13-16 https://doi.org/10.5478/MSL.2015.6.1.13
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Abstract

This study demonstrated the stabilizing role of a cyclodextrin on Keggin [PW12O40]3- via hydrogen bonding complexationunveiled by ESI-MS. The distinctive fragmentation pathways of the {PW12}/γ-CD complexes from that of discrete[PW12O40]3- showed that the so-called “weak” non-covalent interactions can effectively change the dissociation chemistry ofPOM in the gas phase. The influence of different types of solvents and organic additives such as γ-CD on the stability of Keggin[PW12O40]3- was also addressed firstly by ESI-MS.

Kohtaro Sugahara(Suntory Institute for Bioorganic Research) ; Manabu Horikawa(Suntory Institute for Bioorganic Research) ; Tohru Yamagaki(Suntory Institute for Bioorganic Research) pp.17-20 https://doi.org/10.5478/MSL.2015.6.1.17
초록보기
Abstract

Sesamin, one of the lignans in sesame seed, was a labile compound in MS and it was reported that the protonated moleculeof sesamin decomposed easily in ES ionization process and it cannot be detected (G. Yan, et al., Rapid Commun MassSpectrom. 2007, 21, 3613-3620). To protect labile compounds, an amino-cyclodextrin (NCyD) was added to the sample to promotethe host-guest interaction complex in ESI-MS. As a result, sesamin was ionized as the NCyD-sesamin-NCyD (1:2) complexwithout undesired decomposition, suggesting that the amino-CyDs assist the ionization of the labile molecules capped withCyDs by host-guest interaction and these compounds were ionized without their decomposition, those are like amino-CyD complex-assisted ionization. The amino-CyD complexes of sesamin and sesamolin were also analyzed by their ion-mobility MS.

Shuichi Shimma(National Cancer Center Research Institute) pp.21-25 https://doi.org/10.5478/MSL.2015.6.1.21
초록보기
Abstract

In this paper, I describe the importance of matrix spraying conditions in imaging mass spectrometry (IMS) to obtainsuccessful imaging results. My developed matrix application methodology, which is a “two-step matrix application” sequentiallycombined with matrix sublimation and spraying matrix solution can provide high reproducibility and high ion yield comparedwith a conventional direct spraying method. However, insufficient IMS results were obtained occasionally despite the two-stepmethod. Therefore, I wanted to characterize the methodology to continuously provide high quality data. According to my results,the sublimation time was not a strict parameter, and the most important step was the first spraying condition. This means that theextraction conditions from the tissue section and co-crystallization of the matrix were the most important factors.

Mass Spectrometry Letters