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- E-ISSN 2093-8950
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A Fast Determination of Globotriaosylsphingosine in Plasma for Screening Fabry Disease Using UPLC-ESI-MS/MS
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2015, v.6 no.4, pp.116-119
https://doi.org/10.5478/MSL.2015.6.4.116
(Duksung Women’s University)
Yoon,
H.
(2015). A Fast Determination of Globotriaosylsphingosine in Plasma for Screening Fabry Disease Using UPLC-ESI-MS/MS. Mass Spectrometry Letters, 6(4), 116-119, https://doi.org/10.5478/MSL.2015.6.4.116
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Abstract
Globotriaosylsphingosine (lyso-Gb3) is considered as one of the biological marker for Fabry disease. To date, a reli-able biomarker that reflects disease severity and progression has not been discovered to guide the management of Fabry disease. A new method included a simple protein precipitation with acetonitrile in 100 µL of plasma following analyte separation on an Phenomenex Kintex- C18 column using a gradient elution (0.1% formic acid in 5-90% acetonitrile). Total run time was within 12 min including sample preparation and MS/MS analysis. The limit of detection and limit of quantitation were 1 ng/mL and 2 ng/mL, respectively. The calibration curve was linear over the concentration range of 2.0-200.0 ng/mL (r 2 = 0.9999). Inter-day accuracy and precision at 7 level were 93.4-100.7% with RSD of 0.55-5.97%. Absolute recovery was 97.6-98.6%. The method was applied to human and mice plasma, proved the suitability for quantification of lyso-Gb3 for screening, diagnosis and thera-peutic monitoring of Fabry disease patients.
- keywords
- Fabry disease, Globotriaosylsphingosine, Ultraperformance liquid chromatography tandem-mass spectrometry, Inherited metabolic disease.
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- Submission Date
- 2015-11-23
- Revised Date
- 2015-11-25
- Accepted Date
- 2015-11-25
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