- P-ISSN 2233-4203
- E-ISSN 2093-8950
- 권한신청
- P-ISSN2233-4203
- E-ISSN2093-8950
논문 상세
Home > 논문 상세
LC-MS/MS Method for Simultaneous Analysis of Growth Hormone-Releasing Peptides and Secretagogues in Human Urine
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2016, v.7 no.3, pp.55-63
https://doi.org/10.5478/MSL.2016.7.3.55
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
(Korea Institute of Science and Technology)
Min,
H.,
Han,
B.,
Sung,
C.,
Park,
J.,
Lee,
K. M.,
Kim,
H. J.,
Kim,
K. H.,
Son,
J.,
Oh-SeungKwon,
,
&
Jaeick,
L.
(2016). . Mass Spectrometry Letters, 7(3), 55-63, https://doi.org/10.5478/MSL.2016.7.3.55
- 다운로드 수
- 조회수
Abstract
Growth hormone (GH)-releasing peptides (GHRPs) and GH secretagogues (GHSs) are listed in the World Anti-Doping Agency (WADA) Prohibited List. In the present study, we developed and validated a method for the simultaneous analysis of seven GHRPs (alexamorelin, GHRP-1, -2, -4, -5, -6, and hexarelin) and three GHSs (anamorelin, ibutamoren, and ipamorelin) in human urine. Method validation was performed at minimum required performance levels specified by WADA technical documents (2 ng/mL) for all substances, and the method was validated with regard to selectivity (no interference), linearity (R2 > 0.9986), matrix effects (50.0%-141.2%), recovery (10.4%-100.8%), and intra- (2.8%-16.5%) and inter-day (7.0%-22.6%) precisions. The limits of detection for screening and confirmation were 0.05-0.5 ng/mL and 0.05-1 ng/mL, respectively.
- keywords
- Doping control analysis, Growth hormone, GHRP, GHS, Method validation
참고문헌
1
Saugy, M.. (2006). . Brit J. Sport Med, 40(Suppl 1), i35-.
2
Saugy, M.. (1996). . J. Chromatogr. B, 687, 201-.
3
Baumann, G. P.. (2012). . Endocr. Rev, 33, 155-.
4
Bowers, C. Y.. (1991). . Endocrinology, 128, 2027-.
5
Arvat, E.. (2001). . J. Clin. Endocr. Metab, 86, 1169-.
6
Okano, M.. (2010). . Drug Test Anal, 2, 548-.
7
Garcia, J. M.. (2009). . Growth Horm. Igf. Res, 19, 267-.
8
Svensson, J.. (2003). . Growth Horm Igf. Res, 13, 1-.
9
Thomas, A.. (2011). . Anal. Bioanal. Chem, 401, 507-.
10
Okano, M.. (2010). . Rapid Commun. Mass Spectrom, 24, 2046-.
11
Thomas, A.. (2012). . Anal. Chem, 84, 10252-.
12
Sobolevsky, T.. (2014). . Drug Test Anal, 6, 1087-.
13
Mazzarino, M.. (2015). . Forensic Toxicol, 33, 321-.
14
World Anti-Doping Agency. (2015). Technical Document for minimum required performance levels. https://www.wada-ama.org/en/resources/science-medicine/td2015-mrpl.
- 투고일Submission Date
- 2016-08-11
- 수정일Revised Date
- 2016-09-29
- 게재확정일Accepted Date
- 2016-09-29
권호 내 다른 논문
- LC-MS/MS Method for Simultaneous Analysis of Growth Hormone-Releasing Peptides and Secretagogues in Human Urine
- Complete Simultaneous Analysis of Uranium Isotopes in NUSIMEP-7 Microparticles Using SEM-TIMS
- Identification of ML106 Phase 1 Metabolites in Human Liver Microsomes Using High-Resolution Quadrupole-Orbitrap Mass Spectrometry
- Differentiation of Glycan Diversity with Serial Affinity Column Set (SACS)
- Preparation and Characterization of Quercetin-Loaded Solid Dispersion by Solvent Evaporation and Freeze-Drying Method
- more
추천 논문
학술지 홈페이지 이용 환경은 크롬 브라우저 사용을 권장합니다.