- P-ISSN 2233-4203
- E-ISSN 2093-8950
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- P-ISSN2233-4203
- E-ISSN2093-8950
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Free Radical Initiated Peptide Sequencing Using MALDI-TOF/TOF Mass Spectrometry
Mass Spectrometry Letters / Mass Spectrometry Letters, (P)2233-4203; (E)2093-8950
2018, v.9 no.2, pp.56-60
https://doi.org/10.5478/MSL.2018.9.2.56
(Sogang University)
(Sogang University)
(DiatechKorea)
(Hankuk University of Foreign Studies)
(Gachon University)
(Sogang University)
(Sogang University)
(DiatechKorea)
(Hankuk University of Foreign Studies)
(Gachon University)
(Sogang University)
Song,
I., Lee,
J., Baek,
J., Cha,
S., Han,
S. Y., &
Oh,
H.
B.
(2018). Free Radical Initiated Peptide Sequencing Using MALDI-TOF/TOF Mass Spectrometry. Mass Spectrometry Letters, 9(2), 56-60, https://doi.org/10.5478/MSL.2018.9.2.56
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Abstract
In this study, matrix-assisted laser desorption/ionization (MALDI) was applied to the TEMPO-assisted FRIPS for the first time. We found that 3-HPA is the optimal matrix for the analysis of p-TEMPO-Bz-Sc-peptides, which gives minimal precursor fragmentations. MALDI-TOF/TOF experiments on p-TEMPO-Bz-Sc-peptides yielded mainly [a n +H] + , [z n +H] + , and [y n ] + -type products, indicating that radical-driven peptide fragmentation occurs in MALDI-TOF/TOF-MS.
- keywords
- FRIPS, MALDI-TOF/TOF, mass spectrometry, peptides, radical ion, radical-driven fragmentation
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- Submission Date
- 2018-06-02
- Revised Date
- 2018-06-12
- Accepted Date
- 2018-06-12
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